Chromogenic substrates for the assay of β-galactosidase (EC 3.2.1.23)

β-galactosidase is also known as:

β-galactosidase is an enzyme which catalyses the hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides by cleaving a β-glycosidic bond. Assay of β-galactosidase activity can be used for the rapid detection of lactose-fermenting coliform bacteria, including E. coli. In molecular biology, measurement of the levels of active β-galactosidase may be used to monitor the success of gene transfer where the lacZ gene, the gene for β-galactosidase, is used as a reporter gene. 

Enzyme Substrate Product Code MSDS
β-galactosidase MNP-Gal 1053  MNP-Gal
VQM-Gal 1054  VQM-Gal
VBzTM-Gal 1056  VBzTM-Gal
VLE-Gal 1071  VLE-Gal
VLPa-Gal 1072  VLPa-Gal
VLPr-Gal 1073  VLPr-Gal
VQE-Gal 1074  VQE-Gal

Available in 1g, 0.5g, 0.1g quantities. Details of other quantities available on request.

References: Bainbridge et al (1991) Improved method for the detection of β-galactosidase activity in colonies of E. coli using a new chromogenic substrate. FEMS Micobiol Lett. 80, 319-324. Posci et al (1993) Comparison of several new galactosidase as substrates for various β-D-galactosidases. Biochim Biophys Acta, 1163, 54-60.

1053 MNP-Gal powder

This substrate is a pale yellow powder. Hydrolysis of this substrate by β-galactosidase releases a product that is red in colour at pH> 9.5 and can be measured at 505 nm. Extinction coefficient of the released product is 27,000 Lmol-1cm-1.

3-Methoxy-4-(2-nitrovinyl)-phenyl-β-D-galactopyranoside: C15H20NO9
Molecular weight: 357 gmol-1     CAS number: 70622-78-5

MSDS – MNP-Gal

 

1054 VQM-Gal powder

This substrate is an orange yellow powder. Hydrolysis of this substrate by β-galactosidase releases a product that is red in colour at pH> 9.5 and can be measured at 515 nm. Extinction coefficient of the released product is 42,500 Lmol-1cm-1. 

2-{2-[(4-(β-D-galactopyranosyloxy)-3-methoxyphenyl]-vinyl}-1-methylquinolinium iodide: C25H28NO7.I
Molecular weight: 581 gmol-1

MSDS – VQM-Gal

1056 VBzTM-Gal powder

This substrate is an orange yellow powder. Hydrolysis of this substrate by β-galactosidase releases a product that is red in colour at pH> 9.5 and can be measured at 519 nm. Extinction coefficient of the released product is 55,000 Lmol-1cm-1.

2-{2-[4-(β-D-galactopyranosyloxy)-3-methoxyphenyl]-venyl}-3-methylbenzothiazolium iodide: C23H26NO7S.I       
Molecular weight
: 587 gmol-1

MSDS – VBzTM-Gal

1071 VLE-Gal powder

This substrate is an orange yellow powder. Hydrolysis of this substrate by β-galactosidase releases a product that is violet in colour at pH> 9.5 and can be measured at 540 nm. Extinction coefficient of the released product is 33,800 Lmol-1cm-1.

(E)-1-ethyl-4-(3-methoxy-4-(3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yloxy)syryl)quinolinium: C26H30NO7
Molecular weight:
468 gmol-1

MSDS – VLE-Gal

1072 VLPa-Gal powder

This substrate is an orange powder. Hydrolysis of this substrate by β-galactosidase releases a product that is magenta in colour at pH> 9.5 and can be measured at 525 nm. Extinction coefficient of the released product is 35,000 Lmol-1cm-1.

Ammonioum-4-{2-[(4-(β-D-galactopyranosyloxy)-3-methoxyphenyl]-vinyl}-1-(propan-3-yl-carboxylate)-quinolinium: C27H30NO9.Br       
Molecular weight:
592 gmol-1

MSDS – VLPa-Gal

 

1073 VLPr-Gal powder

This substrate is an orange yellow powder. Hydrolysis of this substrate by β-galactosidase releases a product that is violet in colour at pH> 9.5 and can be measured at 555 nm. Extinction coefficient of the released product is 33,800 Lmol-1cm-1.

((E)-4-(3-methoxy-4-(3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yloxy)styryl)-1-propylquinolinium iodide: C27H32NO7.I          
Molecular weight:
609 gmol-1

MSDS – VLPr-Gal

1074 VQE-Gal powder

This substrate is an orange powder. Hydrolysis of this substrate by β-galactosidase releases a product that is magenta in colour at pH> 9.5 and can be measured at 515 nm. Extinction coefficient of the released product is 48,000 Lmol-1cm-1.

1-ethyl-2-(3-methoxy-4-((4R,5R))-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahrdo-2H-pyran-2-yloxy)styryl)quinolinium iodide: C26H30O7N.I          
Molecular weight:
595 gmol-1

MSDS – VQE-Gal