Chromogenic substrates for the assay of Esterase and Lipase (EC 3.1)

Esterases are enzymes that facilitate the hydrolysis of esters into an alcohol and an acid by catalysing the ester bond. Lipases are a subclass of the esterases that can be found in microorganisms, plants or animal tissues. They catalyse esters to get a fatty acid and an alcohol.

Enzyme	Substrate	Product Code	MSDS
Esterase/lipase	MNP-Acetate	1061	MNP-acetate
	MNP-proprionate	1062	MNP-Propionate
	SRA-proprionate	1063	SRA-Propionate
	SRA-butyrate	1064	SRA-Butyrate
	SRA-decanoate	1065	SRA-Decanoate
	SRA-laurate	1066	SRA-Laurate
	SRA-myristate	1067	SRA-Myristate

1061 MNP-Acetate powder

This substrate is a yellow powder. Hydrolysis of this substrate by esterase releases a product that is red in colour at pH> 9.5 and can be measured at 505 nm. Extinction coefficient of the released  product is 27,000 Lmol^-1cm^-1.

2-methoxy-4-(2-nitrovinyl)-phenyl-acetate: C₁₁H₁₁NO₅
Molecular weight: 237 gmol^-1         CAS number: 6622-95-3

MSDS – MNP-Acetate

1062 MNP-Proprionate powder

This substrate is a yellow powder. Hydrolysis of this substrate by esterase releases a product that is red in colour at pH> 9.5 and can be measured at 505 nm. Extinction coefficient of the released  product is 27,000 Lmol^-1cm^-1.

2-methoxy-4-(2-nitrovinyl)-phenyl-propionate: C₁₂H₁₃NO₅
Molecular weight: 251 gmol^-1         CAS number: 77913-02-1

MSDS – MNP-Propionate

1063 SRA-Proprionate powder

This substrate is a yellow powder. Hydrolysis of this substrate by esterase releases a product that is red in colour at pH> 9.5 and can be measured at 529 nm. Extinction coefficient of the released product is 27,700 Lmol^-1cm^-1.

2-[5-[(3,5-dimethoxy-4-propanoyloxy-phenyl)methylene]-4-oxo-2-thioxo-thiazolidin-3-yl]acetic acid: C17H17NO7S₂      
Molecular weight: 411 gmol-1

MSDS – SRA-Propionate

1064 SRA-Butyrate powder

This substrate is a yellow powder. Hydrolysis of this substrate by esterase releases a product that is red in colour at pH> 9.5 and can be measured at 529 nm. Extinction coefficient of the released product is 27,700 Lmol^-1cm^-1.

2-[(5Z)-5-[(4-butanoyloxy-3,5-dimethoxy-phenyl)methylene]-4-oxo-2-thioxo-thiazolidin-3-yl]acetic acid: C18H19NO7S2      
Molecular weight: 425 gmol-1

MSDS – SRA-Butyrate

1065 SRA-Decanoate powder

This substrate is a yellow powder. Hydrolysis of this substrate by esterase/lipase releases a product that is red in colour at pH> 9.5 and can be measured at 529 nm. Extinction coefficient of the released product is 27,700 Lmol^-1cm^-1.

2-[(5Z)-5-[(4-decanoyloxy-3,5-dimethoxy-phenyl)methylene]-4-oxo-2-thioxo-thiazolidin-3-yl]acetic acid: C₂₄H₃₁NO₇S₂
Molecular weight: 509 gmol^-1

MSDS – SRA-Decanoate

1066 SRA-Laurate powder

This substrate is a yellow powder. Hydrolysis of this substrate by esterase/lipase releases a product that is red in colour at pH> 9.5 and can be measured at 529 nm. Extinction coefficient of the released product is 27,700 Lmol^-1cm^-1.

2-[(5Z)-5-[(4-dodecanoyloxy-3,5-dimethoxy-phenyl)methylene]-4-oxo-2-thioxo-thiazolidin-3-yl]acetic acid: C26H35NO7S2      
Molecular weight: 538 gmol-1

MSDS – SRA-Laurate

1067 SRA-Myristate powder

This substrate is a yellow powder. Hydrolysis of this substrate by esterase/lipase releases a product that is red in colour at pH> 9.5 and can be measured at 529 nm. Extinction coefficient of the released product is 27,700 Lmol^-1cm^-1.

2-(5-(3,5-dimethoxy-4-(tetradec-1-en-2-ylperoxy)benzylidene)-4-oxo-2-thioxothiazolidin-3-yl)acetic acid: C₂₈H₃₉NO₇S₂
Molecular weight: 565 gmol^-1

MSDS – SRA-Myristate