Chromogenic substrates for the assay of β-N-acetyl-glucosaminidase (EC 3.2.1.52)

β-N-acetylglucosaminidase is also known as N-acetyl-beta-hexosaminidase, NAG or HEX

In mammals, β-N-acetylglucosaminidase (NAG) is a lysosomal enzyme which catalyses the breakdown of gangliosides and other related glycosides. The assay of urinary NAG activity has been used for the diagnosis of kidney damage and diabetic complications. Elevated serum NAG activity is also associated with some cancers. It is more sensitive than LDH in tissue culture.

Enzyme Substrate Product Code MSDS
β-glucosaminidase/β-hexosaminidase

 

 

 

MNP-GlcNAc 1050  MNP-GlcNAc
VRA-GlcNAc 1051  VRA-GlcNAc
VLM-GlcNAc 1052  VLM-GlcNAc
VLPa-GlcNAc 1070  VLPa-GlcNAc

These products are available in 1g, 0.5g, 0.1g quantities. Details of other quantities available on request.

References: Aamlid et al (1989) Development of improved chromogenic substrates for the detection and assay of hydrolytic enzymes. Chem & In., 106-108. Aamlid et al., (1990) New Colorimetric substrates for the assay of glycosidases. Carbohydrate Research, 205, C5-C9.  Posci et al (1990) VRA-GlcNAc: Novel subsite for N-acetyl-β-D-glucosaminidase applied to the assay of this enzyme in urine. Clin. Chem. 36, 1884-1888.

1050 MNP-GlcNAc powder

This substrate is a pale-yellow powder which on hydrolysis by β-N-acetylglucosaminidase releases a soluble product that is red at pH> 9.5 and is measured at 505 nm. The Extinction coefficient of the released chromogenic product is 27,000 Lmol-1cm-1

2-Methoxy-4-(2-nitrovinyl)-phenyl-2-acetamido-2-deoxy-β-D-glucopyranoside: C17H22O9N2
Molecular weight: 398 gmol-1       CAS number: 70622-74-1

MSDS – MNP-GlcNAc

1051 VRA-GlcNAc powder

This substrate is a bright yellow powder. Hydrolysis of this substrate by β-N-acetylglucosaminidase releases a product that is red in colour at pH> 9.5 and can be measured at 492 nm. Extinction coefficient of the released chromogenic product is 37,000 Lmol-1cm-1.

Ammonium-5-[4-(2-acetamido-2-deoxy-beta-D-glucopyranosyloxy)-3-methoxymethylene]-2-thioxothiazolidin-4-one-3-acetate hydrate: C21H27N3O10S2     
Molecular weight: 546 gmol-1      CAS number: 125261-87-2

MSDS – VRA-GlcNAc

1052 VLM-GlcNAc powder

This chromogenic substrate is an orange powder. Hydrolysis of this substrate by β-N-acetylglucosaminidase releases a product that is magenta in colour at pH> 9.5 and can be measured at 524 nm. Extinction coefficient of the released chromogenic product is 35,000 Lmol-1cm-1.

4-{2-[4-(2-Acetamido-2-deoxy-beta-D-glucopyranosyloxy)-3-methoxyphenyl]-vinyl}-1-methyl-quinolinium iodide: C27H31N2O7.I        
Molecular weight
: 622 gmol-1

MSDS – VLM-GlcNAc

1070 VLPa-GlcNAc powder

This chromogenic substrate is an orange powder. Hydrolysis of this substrate by β-N-acetylglucosaminidase releases a product that is magenta in colour at pH> 9.5 and can be measured at 525 nm. Extinction coefficient of the released chromogenic product is 35,000 Lmol-1cm-1.

4-{2-[4-(2-Acetamido-2-deoxy-beta-D-glucopyranosyloxy)-3-methoxyphenyl]-vinyl}-1-propan-3-oate-quinolinium bromide: C29H34N3O9.Br      
Molecular weight: 648 gmol-1

MSDS – VLPa-GlcNAc