NAG Products

MNP-NAG Assay Kits

Calibrant/Colorimetric Assay Method for the determination of NAG (N-Acetyl-β-D-glucosaminidase)

Description MSDS Product Code
300 test kit or 50 manual test kit* 1085
300 test kit (equivalent to 50 manual tests) 1290050
600 test kit (equivalent to 100 manual tests) 1290100
Calibrant 1290011

*Unlike 1290100 and 1290050 kits, Buffer A is divided into five bottles, this allows small batches of samples to be run at different times and is ideal for initial studies as N + A solution once mixed is only stable for 5 days.

The company also offers highly sensitive alternatives to the above MNP-NAG kits. See VRA-NAG assay kit (spectrophotometric method).

Introduction to the NAG Kit

The NAG kit (MNP-NAG assay kit) is a sensitive, non-invasive quantitative test. N-acetyl-β-D-glucosaminidase (NAG) is a well-recognised early marker for renal damage including Acute kidney injury (AKI). The kit can be used to measure NAG in urine or serum samples.

NAG is measured in urine as a well-established, non-invasive marker of AKI, nephrotoxicity and the progression of renal disease. Renal disease is progessive resulting in deterioration of renal function. This evertually results in renal failure which necessitates either renal dialysis or transplantation.

Hexosaminidase (HEX) isoenzymes are present in different organs and tissues. The acidic A and basic B forms are present in the liver, kidney and spleen. HEX is also assayed in laboratories monitoring genetic disease in suspected cases of Tay-Sach disease and Sandhoff’s disease. The A-form of HEX is absent from Tay-Sach disease which is most commonly found in Ashkenasi Jews. This kit can be used in conjunction with heat treatment or ion-exchange chromatography in the quantitation of the A and B-forms of hexosaminidase.

Application

The enzyme is assayed using the company’s Rapichrome Solution N, containing 2-Methoxy-4-(2-nitrovinyl)-phenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (MNP-Glc-NAc). The reaction takes place at 37°C and is stopped by using the alkaline Stopping Buffer G, giving a colour change. The absorbance is read at 505nm. The final value is calculated using reference value provided with our Calibrants.

Find out how to use our NAG Kit

Key Features

  • Well established:  NAG is a well established accurate early marker of acute kidney injury (AKI) and renal damage
  • High Specificity: Test is highly specific for detection of NAG enzyme from only 50µl of urine sample.
  • Adaptability: Colorimetric procedure is adaptable to any laboratory and can be used with automated analyzers as well as manual spectro-photometric detection. Look at the available method sheets below.
  • Can be used in conjunction with heat treatment or ion-exchange chromatography in the quantitation of the A and B-forms of hexosaminidase
  • 3 Kits available in various sizes to suit type of investigation

NAG leaflet folded PDF-2016