Chromogenic substrates for the assay of Esterase and Lipase (EC 3.1)

Applications

Esterases are enzymes that facilitate the hydrolysis of esters into an alcohol and an acid by catalysing the ester bond. Lipases are a subclass of the esterases that can be found in microorganisms, plants or animal tissues. They catalyse esters to get a fatty acid and an alcohol.

1061 MNP-Acetate powder

2-methoxy-4-(2-nitrovinyl)-phenyl-acetate

Formula: C11H11NO5       Molecular weight: 237 gmol-1         CAS number: 6622-95-3

MSDS sheet

Description

This chromogenic substrate is supplied as a yellow powder. Hydrolysis of this substrate by esterase releases a soluble end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 505 nm. Extinction coefficient of the released chromogenic product is 27,000 Lmol-1cm-1.

1062 MNP-Proprionate powder

2-methoxy-4-(2-nitrovinyl)-phenyl-propionate

Formula: C12H13NO5       Molecular weight: 251 gmol-1         CAS number: 77913-02-1

MSDS sheet

Description

This chromogenic substrate is supplied as a yellow powder. Hydrolysis of this substrate by esterase releases a soluble end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 505 nm. Extinction coefficient of the released chromogenic product is 27,000 Lmol-1cm-1.

1063 SRA-Proprionate powder

2-[5-[(3,5-dimethoxy-4-propanoyloxy-phenyl)methylene]-4-oxo-2-thioxo-thiazolidin-3-yl]acetic acid

Formula: C17H17NO7S2       Molecular weight: 411 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as a yellow powder. Hydrolysis of this substrate by esterase releases a soluble end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 529 nm. Extinction coefficient of the released chromogenic product is 27,700 Lmol-1cm-1.

1064 SRA-Butyrate powder

2-[(5Z)-5-[(4-butanoyloxy-3,5-dimethoxy-phenyl)methylene]-4-oxo-2-thioxo-thiazolidin-3-yl]acetic acid

Formula: C18H19NO7S2       Molecular weight: 425 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as a yellow powder. Hydrolysis of this substrate by esterase releases a soluble end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 529 nm. Extinction coefficient of the released chromogenic product is 27,700 Lmol-1cm-1.

1065 SRA-Decanoate powder

2-[(5Z)-5-[(4-decanoyloxy-3,5-dimethoxy-phenyl)methylene]-4-oxo-2-thioxo-thiazolidin-3-yl]acetic acid

Formula: C24H31NO7S2       Molecular weight: 509 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as a yellow powder. Hydrolysis of this substrate by esterase/lipase releases a soluble end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 529 nm. Extinction coefficient of the released chromogenic product is 27,700 Lmol-1cm-1.

1066 SRA-Laurate powder

2-[(5Z)-5-[(4-dodecanoyloxy-3,5-dimethoxy-phenyl)methylene]-4-oxo-2-thioxo-thiazolidin-3-yl]acetic acid

Formula: C26H35NO7S2       Molecular weight: 538 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as a yellow powder. Hydrolysis of this substrate by esterase/lipase releases a soluble end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 529 nm. Extinction coefficient of the released chromogenic product is 27,700 Lmol-1cm-1.

1067 SRA-Myristate powder

2-(5-(3,5-dimethoxy-4-(tetradec-1-en-2-ylperoxy)benzylidene)-4-oxo-2-thioxothiazolidin-3-yl)acetic acid

Formula: C28H39NO7S2       Molecular weight: 565 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as a yellow powder. Hydrolysis of this substrate by esterase/lipase releases a soluble end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 529 nm. Extinction coefficient of the released chromogenic product is 27,700 Lmol-1cm-1.

Enzyme Substrate Product Code MSDS
Esterase/lipase MNP-Acetate 1061
MNP-proprionate 1062
SRA-proprionate 1063
SRA-butyrate 1064
SRA-decanoate 1065
SRA-laurate 1066
SRA-myristate 1067