Chromogenic substrates for the assay of alkaline phosphatase (EC 3.1.3.1) and acid phosphatase (EC 3.1.3.2)

Alkaline phosphatase is also known as:

Basic phosphatase; alkaline phosphomonoesterase; phosphomonoesterase; glycerophosphatase; alkaline phosphohydrolase; alkaline phenyl phosphatase; orthophosphoric-monoester phosphohydrolase (alkaline optimum); ALP; ALKP; ALPase; Alk Phos.

Acid phosphatase is also known as:

Acid phosphomonoesterase; phosphomonoesterase; glycerophosphatase; acid monophosphatase; acid phosphohydrolase; acid phosphomonoester hydrolase; uteroferrin; acid nucleoside diphosphate phosphatase; orthophosphoric-monoester phosphohydrolase (acid optimum); AP; ACP.

Applications

Phosphatases are hydrolase enzymes which remove phosphate group from other molecules. Measurement of phosphatase activity can be performed to detect phosphatase-active bacteria. In humans, elevated serum alkaline phosphatase levels have been associated with various diseases, such as biliary obstruction, bone conditions, liver diseases and leukemia.

1068 SLPr-Phos powder

[2,6-dimethoxy-4-[(E)-2-(1-propylquinolin-1-ium-4-yl)vinyl]phenyl] hydrogen phosphate pyridin-1-ium iodide

Formula: C27H30N2O6P.I            Molecular weight: 636 gmol-1

MSDS – SLPr-Phos

Description

This chromogenic substrate is supplied as an orange brown powder. Hydrolysis of this substrate by phosphatase releases a soluble end product that is violet/blue in colour at pH> 9.5 and can be measured spectrophotometrically at 550 nm. Extinction coefficient of the released chromogenic product is 17,000 Lmol-1cm-1.

Enzyme Substrate Product Code MSDS
Phosphatase SLPr-Phos 1068  SLPr-Phos

Available in 1g, 0.5g, 0.1g quantities. Details of other quantities available on request.