Chromogenic substrates for the assay of β-glucosidase (EC 3.2.1.21)

β-glucosidase is also known as:

Beta-glucosidase; amygdalase; beta-D-glucoside glucohydrolase; cellobiase; gentobiase.

Applications

β-glucosidase is an enzyme which catalyses the hydrolysis of carbohydrates by cleaving a β1→4 bond to release a terminal non-reducing β-D-glucosyl residue. Cellulolytic organisms (some fungi and bacteria) possess β-glucosidase for the breakdown of cellulose as carbon sources. In humans, deficiencies in acid β-glucosidase have been implicated in Gaucher’s disease and Parkinson disease.

1057 VQM-Glc powder

2-{2-[(4-(β-D-glucopyranosyloxy)-3-methoxyphenyl]-vinyl}-1-methylquinolium iodide

Formula: C25H28NO7.I             Molecular weight: 581 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as a greenish yellow powder. Hydrolysis of this substrate by β-glucosidase releases a soluble end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 515 nm. Extinction coefficient of the released chromogenic product is 42,500 Lmol-1cm-1.

1059 VBzTM-Glc powder

2-{2-[(4-(β-D-glucopyranosyloxy)-3-methoxyphenyl]-vinyl}-3-methylbenzothiazolium iodide

Formula: C23H30NO7S.I          Molecular weight: 587 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as an orange yellow powder. Hydrolysis of this substrate by β-glucosidase releases an end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 519 nm. Extinction coefficient of the released chromogenic product is 55,000 Lmol-1cm-1.

1060 VRA-Glc powder

Ammonium-5-[4-(β-D-glucopyranosyloxy)-3-methoxymethylene]-2-thioxothiazolidin-4-one-3-acetate

Formula: C19H20NO10S2.H4N     Molecular weight: 504 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as a bright yellow powder. Hydrolysis of this substrate by β-glucosidase releases an end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 492 nm. Extinction coefficient of the released chromogenic product is 37,000 Lmol-1cm-1.

Enzyme Substrate Product Code MSDS
β-glucosidase

 

 

 

VQM-Glc 1057  
VBzTM-Glc 1059  
VRA-Glc 1060  

These products are available in 1g, 0.5g, 0.1g quantities. Details of other quantities available on request.