Chromogenic substrates for the assay of β-galactosidase (EC 3.2.1.23)

β-galactosidase is also known as:

Beta-galactosidase; lactase; β-lactosidase; maxilact; hydrolact; β-D-lactosidase; S 2107; lactozym; trilactase; β-D-galactanase; oryzatym; sumiklat; β-D-galactoside galactohydrolase; beta-gal; β-gal.

Applications

β-galactosidase is an enzyme which catalyses the hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides by cleaving a β-glycosidic bond. Assay of β-galactosidase activity can be used for the rapid detection of lactose-fermenting coliform bacteria, including E. coli. In molecular biology, measurement of the levels of active β-galactosidase may be used to monitor the success of gene transfer where the lacZ gene, the gene for β-galactosidase, is used as a reporter gene. 

1053 MNP-Gal powder

3-Methoxy-4-(2-nitrovinyl)-phenyl-β-D-galactopyranoside

Formula: C15H20NO9       Molecular weight: 357 gmol-1     CAS number: 70622-78-5

MSDS sheet

Description

This chromogenic substrate is supplied as a pale yellow powder. Hydrolysis of this substrate by β-galactosidase releases a soluble end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 505 nm. Extinction coefficient of the released chromogenic product is 27,000 Lmol-1cm-1.

1054 VQM-Gal powder

2-{2-[(4-(β-D-galactopyranosyloxy)-3-methoxyphenyl]-vinyl}-1-methylquinolinium iodide

Formula: C25H28NO7.I            Molecular weight: 581 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as an orange yellow powder. Hydrolysis of this substrate by β-galactosidase releases an end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 515 nm. Extinction coefficient of the released chromogenic product is 42,500 Lmol-1cm-1. 

1056 VBzTM-Gal powder

2-{2-[4-(β-D-galactopyranosyloxy)-3-methoxyphenyl]-venyl}-3-methylbenzothiazolium iodide

Formula: C23H26NO7S.I          Molecular weight: 587 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as an orange yellow powder. Hydrolysis of this substrate by β-galactosidase releases an end product that is red in colour at pH> 9.5 and can be measured spectrophotometrically at 519 nm. Extinction coefficient of the released chromogenic product is 55,000 Lmol-1cm-1.

1071 VLE-Gal powder

(E)-1-ethyl-4-(3-methoxy-4-(3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yloxy)syryl)quinolinium

Formula: C26H30NO7             Molecular weight: 468 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as an orange yellow powder. Hydrolysis of this substrate by β-galactosidase releases an end product that is violet in colour at pH> 9.5 and can be measured spectrophotometrically at 540 nm. Extinction coefficient of the released chromogenic product is 33,800 Lmol-1cm-1.

1072 VLPa-Gal powder

Ammonioum-4-{2-[(4-(β-D-galactopyranosyloxy)-3-methoxyphenyl]-vinyl}-1-(propan-3-yl-carboxylate)-quinolinium

Formula: C27H30NO9.Br          Molecular weight: 592 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as an orange powder. Hydrolysis of this substrate by β-galactosidase releases an end product that is magenta in colour at pH> 9.5 and can be measured spectrophotometrically at 525 nm. Extinction coefficient of the released chromogenic product is 35,000 Lmol-1cm-1.

1073 VLPr-Gal powder

((E)-4-(3-methoxy-4-(3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yloxy)styryl)-1-propylquinolinium iodide

Formula: C27H32NO7.I             Molecular weight: 609 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as an orange yellow powder. Hydrolysis of this substrate by β-galactosidase releases an end product that is violet in colour at pH> 9.5 and can be measured spectrophotometrically at 555 nm. Extinction coefficient of the released chromogenic product is 33,800 Lmol-1cm-1.

1074 VQE-Gal powder

1-ethyl-2-(3-methoxy-4-((4R,5R))-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahrdo-2H-pyran-2-yloxy)styryl)quinolinium iodide

Formula: C26H30O7N.I            Molecular weight: 595 gmol-1

MSDS sheet

Description

This chromogenic substrate is supplied as an orange powder. Hydrolysis of this substrate by β-galactosidase releases an end product that is magenta in colour at pH> 9.5 and can be measured spectrophotometrically at 515 nm. Extinction coefficient of the released chromogenic product is 48,000 Lmol-1cm-1.

Enzyme Substrate Product Code MSDS
β-galactosidase MNP-Gal 1053  
VQM-Gal 1054  
VBzTM-Gal 1056  
VLE-Gal 1071  
VLPa-Gal 1072  
VLPr-Gal 1073  
VQE-Gal 1074  

Available in 1g, 0.5g, 0.1g quantities. Details of other quantities available on request.